Plant Arginine(Arg)Content HPLC Assay Kit

Product Description L-Arginine is a conditionally essential amino acid; healthy adults can synthesize it endogenously. It plays multiple key roles in vivo: as a precursor of nitric oxide (NO)， L-Arginine dilates blood vessels and improves blood flow through NO production; it participates in the urea cycle， helping to convert toxic ammonia into urea for excretion; it promotes wound healing， enhances immune function， and stimulates the release of growth hormone and insulin. L-Arginine lacks ultraviolet (UV) absorption groups and strong fluorescent properties， and thus cannot be directly determined by conventional detectors. This kit employs a pre-column derivatization method: under weakly alkaline conditions， OPA (o-phthalaldehyde) and 3-MPA (3-mercaptopropionic acid) specifically react with the amino group of L-Arginine to form fluorescent derivatives. Upon excitation with ultraviolet light， these derivatives emit fluorescence at a longer wavelength than the excitation light. The fluorescence intensity is proportional to the L-Arginine concentration with a high response， making this method suitable for automated batch analysis by HPLC. This kit is specifically designed for the rapid and efficient extraction and quantification of L-Arginine in plant samples. Instruments and Reagents Required for the Assay High-performance liquid chromatograph (HPLC) equipped with a C18 column (4.6 × 250 mm， water tolerance ≥95%) and a fluorescence detector (FLD); ultrasonic cleaner; vortex mixer; electronic balance; low-temperature high-speed centrifuge (4°C， 10，000 r/min); mortar/homogenizer; adjustable pipettes; syringe; organic membrane filter; aqueous membrane filter; chromatographic-grade methanol， ethanol， and acetonitrile， etc.