Plant γ-Aminobutyric Acid(GABA)Content HPLC Assay Kit

Product Description γ-Aminobutyric acid (GABA) is a naturally occurring bioactive compound widely distributed in animals and plants. In plants， GABA is found in seeds， roots， rhizomes， and tissue fluids of genera such as Fagopyrum， Panax， and various Chinese herbal medicines. In animals， GABA is mainly present in nervous tissues， where it serves as the primary inhibitory neurotransmitter in the adult mammalian brain. It can block specific signals in the central nervous system and produce a sedative effect. GABA lacks ultraviolet (UV) absorption groups and strong fluorescent properties， and thus cannot be directly determined by conventional detectors. This kit employs a pre-column derivatization method: under weakly alkaline conditions， OPA (o-phthalaldehyde) and 3-MPA (3-mercaptopropionic acid) specifically react with the amino group of GABA to form fluorescent derivatives. Upon excitation with ultraviolet light， these derivatives emit fluorescence at a longer wavelength than the excitation light. The fluorescence intensity is proportional to the GABA concentration with a high response， making this method suitable for automated batch analysis by HPLC. This kit is specifically designed for the rapid and efficient extraction and quantification of GABA in plant samples. Instruments and Reagents Required for the Assay High-performance liquid chromatograph (HPLC) equipped with a C18 column (4.6 × 250 mm， water tolerance ≥95%) and a fluorescence detector (FLD); ultrasonic cleaner; vortex mixer; electronic balance; low-temperature high-speed centrifuge (4°C， 10，000 r/min); mortar/homogenizer; adjustable pipettes; syringe; organic membrane filter; aqueous membrane filter; chromatographic-grade methanol， ethanol， and acetonitrile， etc.