Phosphotransacetylase（PTA）Activity Assay Kit

Determination of Significance: Phosphotransacetylase (PTA, EC 2.3.1.8) is one of the key enzymes related to acetate metabolism. Acetate kinase (ACK) and PTA catalyze acetic acid to generate acetyl coenzyme A, which is an important substance in the metabolism of carbohydrate, fat and protein. Measurement Principle: PTA catalyzes acetyl coenzyme A and phosphate to produce acetyl phosphate and coenzyme A, following the transformation from DTNB to yellow TNB. TNB has a characteristic absorption peak at 412 nm, and PTA activity is calculated by measuring the rate of increase in absorbance at 412nm. Self Provided: Spectrophotometer/Microplate reader, water-bath, desk centrifuge, balance, transferpettor, micro glass cuvette/96-well flat-bottom plate, mortar/homogenizer/cell ultrasonic crusher, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.