Phosphoenolpyruvate Carboxylase(PEPC) Activity Assay Kit

Determination of Significance: Phosphoenolpyruvate carboxylase (EC 4.1.1.31) is widely found in plants and microorganisms. It catalyzes the irreversible reaction of phosphoenolpyruvate and carbon dioxide to oxaloacetic acid. It is also a key enzyme for C4 plants and cam plants to fix CO2. It plays an important role in regulating the operation of tricarboxylic acid cycle. Measurement Principle: PEPC can catalyze phosphoenolpyruvate and carbon dioxide to form oxaloacetate and HPO42-. Malate dehydrogenase can catalyze oxaloacetate and NADH to produce malate and NAD+. The rate of NADH reduction was measured at 340 nm and PEPC activity was calculated. Self Provided: Ultraviolet spectrophotometer/microplate reader, desk centrifuge, water-bath, transferpettor, micro quartz cuvette/96 well UV plate, pipette, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.