Oxidized Thioredoxin Reductase(TrxR) Activity Assay Kit

Determination of Significance: Thioredoxin Reductase (TrxR) is a NADPH-dependent dimer selenase and includes FAD structure domain. TrxR belongs to pyridine nucleotide-disulfide REDOX enzyme, and form thioredoxin system with thioredoxin and NADPH. The activity of TrxR is similar with GR. TrxR could catalyzes GSSG reduct to GSH, which is the key enzyme in glutathione REDOX cycle. Measurement Principle: TrxR catalyzes NADPH to reductDTNB form TNB and NADP+, TNB has a absorbance at 412 nm, but reduced glutathione reacts with DTNB to form TNB, so the 2-Vinylpyridine in this kit can inhibit reduced glutathione in sample. The activity of TrxR can be calculated by detecting increase rate of TNB at 412 nm. Self Provided: Spectrophotometer, low temperature centrifuge, adjustable pipette, 1 mL glass cuvette and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.