O27 Serum-Free Supplement (50×，without Vitamin A)

Make sure low temparature condition during transportation. Storing at -20℃ is recommanded. Thaw at 4℃. Avoid repeated freezing/thawing (no more than twice). Thawed product should not be stored at 4℃ for more than 2 weeks. Using aseptic technique when operating. O27 Supplement is a refined organoid culture supplement, which is specially designed for organoid culture and tested for culture of various organoids and neurons. O27 retains advantages of traditional B27 and add customized nutrients regarding different organoids, therefore enabling more specific and stable organoid/cell culture environment. This product helps researchers simulate human body organ function. O27 cultre supplement is 50× liquid, to use in conjunction with organoid culture base or add into neuron medium base for neuron culture without requiring astrocyte feeder layer.