Nucleus-Cytoplasm Extraction Kit

1. Unopened protease inhibitor can be stored at 2-8 °C; store at -20 °C after opening.2. The protease inhibitor exists as solid at 2-8 °C. Take it out and warm to room temperature or incubate briefly in a 37 °C water bath to liquefy，then centrifuge to collect liquid at tube bottom before opening.3. Use up the reagent promptly once opened. Solarbio Nuclear/Cytoplasmic Extraction Kit enables separation of nuclear and cytoplasmic fractions from mammalian cells. The kit is designed to keep isolated nuclear and cytoplasmic components intact and segregated. Optimized reagents and protocols allow rapid extraction with minimal cross-contamination. Recovered fractions retain native biological activities and are suitable for downstream applications including transcription assay，RNA splicing，EMSA，reporter assay，enzyme activity detection and Western blot.