Nitrate Reductase(NR) Activity Assay Kit

Determination of Significance: NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen into ammonia nitrogen as well as an induction enzyme, widely exists in plants and has an impact on crop yield and quality. Measurement Principle: NR catalyzed nitrate reduction to nitrite, with NO3ˉ + NADH + H＋→ NO2ˉ + NAD＋+ H2O. NADH has a characteristic absorption peak at 340 nm. The change of absorbance at 340 nm can indicate the enzyme activity. Self Provided: Table centrifuge, water-bath, micro quartz cuvette/96 well UV flat-bottom plate, spectrophotometer/ microplate reader, mortar/ homogenate, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.