Nitrate Reductase (NR) Assay Kit（Griess-Colorimetric Method）

Determination of Significance: NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen into ammonia nitrogen as well as an induction enzyme, which widely exists in plants and has an impact on crop yield and quality. Measurement Principle: NR catalyzed nitrate reduction to nitrite, with NO3ˉ +NADH+H＋→NO2ˉ+NAD＋+H2O. In the acidic conditions, the NO2ˉ produced can participate in the diazotization reaction to produce a purple-red compound, which has an absorption peak at 540 nm, and the change in absorbance value at 540 nm indicates the enzyme activity. Self Provided: Spectrophotometer, water bath/incubator, benchtop centrifuge, 1 mL glass cuvette, mortar/ homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.