Nickase Endonuclease Nt.BstNBI

The optimal reaction temperature is 55℃;
The volume of enzyme added to the reaction system should not exceed 10% of the total volume to avoid excessive glycerol in the enzyme causing star-shaped activity;
The additives (e.g., glycerol, salt) in the restriction endonuclease storage buffer are the same as the contaminants (e.g., salt, EDTA, or ethanol) in the substrate solution. The smaller the reaction volume, the stronger the inhibitory effect of the enzyme digestion reaction;
Inactivation conditions: incubation at 80℃ for 20 min. Nt. BstNBI is a nicking endonuclease that cleaves only one strand of a double-stranded DNA (dsDNA) substrate ， creating a nick on dsDNA without separating the two strands. Concentration: 10?U/μLRecognition site: GAGTCNNNNN5 ‘…GAGTCNNNN↓N…3 ‘3 ‘…CTCAGNNNNN…5 ‘Definition of enzyme activity:One unit (U) is defined as the amount of enzyme required to completely convert 1?μg of supercoiled pUC19 DNA into the open-circular form in a 50?μL reaction system at 55?°C within 1 hour.