Nickase Endonuclease Nb.BbvCI

The volume of enzyme added to the reaction system should not exceed 10% of the total volume to avoid excessive glycerol in the enzyme causing star-shaped activity;
The additives (e.g., glycerol, salt) in the restriction endonuclease storage buffer are the same as the contaminants (e.g., salt, EDTA, or ethanol) in the substrate solution. The smaller the reaction volume, the stronger the inhibitory effect of the enzyme digestion reaction;
For DNA methylated by EcoBI, cleavage may be inhibited;
Inactivation conditions: Incubate at 80℃ for 20 min. Nb. BbvCI is a nicking endonuclease that cleaves only one strand of a double-stranded DNA (dsDNA) substrate; it produces a nick on dsDNA without separating the two strands. Nb. BbvCI is commonly used in isothermal nucleic acid amplification (such as SDA and RCA). The nick generated by Nb. BbvCI triggers the strand displacement reaction of DNA polymerase ， and repeated cycles of nicking ， displacement and extension enable exponential amplification of nucleic acids. Concentration: 10?U/μLRecognition site: CCTCAGC5 ‘…CCTCAGC…3 ‘3 ‘…GGAGT↑CG…5 ‘Definition of enzyme activity:One unit (U) is defined as the amount of enzyme required to completely convert 1?μg of supercoiled p615 DNA into the open-circular form in a 50?μL reaction system at 37?°C within 1 hour.