NADH Oxidase(NOX) Activity Assay Kit

Determination of Significance: NADH oxidase (NOX) (EC 1.6.99.3) exists widely in animals, plants, microorganisms and cultured cells, which can direct oxidation of NADH to NAD under oxygen. This enzyme is not only involved in the regeneration of NAD, but also closely related to immune response. Measurement Principle: NOX can oxidize NADH to NAD, oxidation of NADH is coupled to the reduction phase of 2,6-dichlorophenol indigo (DCPIP) and the blue DCPIP is restored to colorless DCPIP. The NOX activity can be quantified by measuring the decrease in the color development at 600 nm. Self Provided: Microplate reader/spectrophotometer, water bath, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.