Solarbio

Mouse TNFRSF1A ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-18 present is captured by the coated antibody after incubation. Following extensive washing, a HRP-conjugate antibody specific for IL-18 is added to detect the captured IL-18 protein in sample. For signal development, horseradish peroxidase (HRP)-conjugated Streptavidin is added, followed by tetramethyl-benzidine (TMB) reagent. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKM-0245-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

Reactivity:

Mouse

Detection Range:

9.38-600 pg/ml

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

4.69 pg/ml

Specificity:

Do not react with Mouse TNF-α、TNF-RI Rat TNF-α Human TNF-α、TNF-β、TNF -RI、TNF-RII etc.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان