کیت
Solarbio

Mouse TNFR2/TNFRSF1B ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative sandwich enzyme immunoassay technique. An antibody specific for mouse TNFR2/TNFRSF1B has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and allow them to fully combine with the specific antibody. After washing away any unbound substances a horseradish peroxidase (HRP)-conjugated antibody specific for mouse TNFR2/TNFRSF1B is added to detect the captured mouse TNFR2/TNFRSF1B protein in the sample. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity proportional to the quantity of bound protein is then measurable at 450nm. You can calculate the concentration of mouse TNFR2/TNFRSF1B in the samples by comparing the O.D. of the samples to the standard curve.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKM-0207-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
3h
Reactivity:
Mouse
Detection Range:
9.375-600 pg/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
4.8 pg/ml
Specificity:
Do not react with C10 Eotaxin G-CSF GM-CSF IFN-γ IL-1α IL-1β IL-1ra IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-9 IL-10 IL-10R IL-12 IL-13 IL-17 IL-18 JE/MCP-1 KC Leptin LIF M-CSF MIP-1α MIP-1β MIP-2 OSM SCF TNF-α TNFRI Tpo VEGF etc.
تحویل:
یک ماه پس از سفارش
80400000
80400000 تومان