Solarbio

Mouse TNF-α(One-Step）ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse TNF-α has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Mouse TNF-α present is captured by the coated antibody after incubation. A biotin-conjugate antibody specific for Mouse TNF-α is added to detect the captured Mouse TNF-α protein in the sample. Horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKM-0034(OS)-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

60min

Reactivity:

Mouse

Detection Range:

31.25-2000 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

15.625 pg/ml

Specificity:

Do not react with Mouse G-CSF,GM-CSF,IL-1α,IL-1β,IL-2,IL-3,IL-5,IL-6,IL-7,IL-9,Human TNF-α,TNF sRI,TNF sRII ect.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان