Mouse Pan Naive T Cell Sorting Kit (Negative Selection)

This kit employs a negative selection approach to isolate Pan Naive T cells from mouse spleens via column-free magnetic sorting. During separation, antibodies and magnetic beads do not touch the T cells, maximally preserving the pristine state of Pan Naive T cells. The kit labels non-Pan Naive T cells with biotin-conjugated antibodies, incubates the cells with streptavidin-magnetic beads for conjugation, and then performs column-free separation via magnetic separation. Target cells can be isolated simply by transferring them to a new tube. The isolated cells are immediately ready for subsequent applications such as flow cytometry, cell culture, and functional assays.