کیت
Solarbio

Mouse M-CSF ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for M-CSF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any M-CSF present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for M-CSF is added to detect the captured M-CSF protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKM-0042-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
3h
Reactivity:
Mouse
Detection Range:
7.81-500 pg/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
3.9 pg/ml
Specificity:
Do not react with Mouse G-CSF&GM-CSF&IFN-γ&IL-1α&IL-1β&IL-2&IL-3&IL-4&IL-5&IL-6&IL-7&IL-9&IL-10&IL-10 R&IL-12 p40&IL-12 p70&IL-13&IL-18 etc.
تحویل:
یک ماه پس از سفارش
80400000
80400000 تومان