Mouse Lymphotactin/XCL1 ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Lymphotactin/XCL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Lymphotactin/XCL1 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Lymphotactin/XCL1 is added to detect the captured Lymphotactin/XCL1 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.