Mouse Intervertebral Disc Annulus Fibrosus Cell Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal mice for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Reagent preparation or aliquoting must strictly adhere to aseptic technique guidelines. After aliquoting, immediately seal the containers with sealing film, use immediately upon removal, and avoid repeated freeze-thaw cycles or contamination. The Mouse Intervertebral Disc Annulus Fibrosus Cell Isolation and Culture Kit is specifically designed for the isolation of primary mouse intervertebral disc annulus fibrosus cells. Upon verification, following the standard operating procedures of this kit, one test can yield one bottle of cells (T25 culture flask), with a cell count exceeding 1×10? cells. When passaged at a 1:2 ratio, the cells can be passaged for 3-5 generations. Immunofluorescence analysis confirms a cell purity (type II collagen) exceeding 90%.