Solarbio

Mouse insulin ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Insulin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Insulin present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Insulin is added to detect the captured Insulin protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKM-0387-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

Reactivity:

Mouse

Detection Range:

12.5-800 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

6.25 pg/ml

Specificity:

This kit recognizes Mouse Insulin in samples. No significant cross-reactivity or interference between Mouse Insulin and analogues was observed.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان