کیت
Solarbio

Mouse IgG(Uncoated) ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgG has been pre-coated onto a microplate.Standards and samples are pipetted into the wells; any IgG present is captured by the coated antibody after incubation. After washing away any unbound substances, a HRP-conjugate antibody specific for IgG is added to detect the captured IgG protein in the sample. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKUM-0001-2*96T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
12.75h
Reactivity:
Mouse
Detection Range:
1.56-100 ng/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
243 pg/ml
Specificity:
Do not react with Mouse IgM、IgA,Human、Porcine、Rabbit、Goat、Sheep、Chicken IgG ect.
تحویل:
یک ماه پس از سفارش
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