کیت
Solarbio

Mouse IFN-β(One-Step)ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse IFN-β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Mouse IFN-β present is captured by the coated antibody after incubation. A biotin-conjugate antibody specific for Mouse IFN-β is added to detect the captured Mouse IFN-β protein in the sample. Horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKM-0032(OS)-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
60min
Reactivity:
Mouse
Detection Range:
25-1600pg/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
12.5pg/ml
Specificity:
Do not react with Mouse IFN-α1,IFN-α/βR2,IFN-κ,IFN-γ,IL-6,IL-28A,Limitin,Human IFN-β1 ect.
تحویل:
یک ماه پس از سفارش
100200000
100200000 تومان