Solarbio

Mouse HMGB1 ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any HMGB1 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for HMGB1 is added to detect the captured HMGB1 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKM-0145-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Reactivity:

Mouse

Detection Range:

0.625-40ng/mL

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

312.5pg/mL

Specificity:

It has 101% homology to human, rat, bovine, and porcine HMGB1

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

100200000 تومان