کیت
Solarbio

Mouse Fas ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Mouse Fas has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Mouse Fas present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Mouse Fas is added to detect the captured Mouse Fas protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKM-0076-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
3h
Reactivity:
Mouse
Detection Range:
62.5-4000 pg/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
31.25 pg/ml
Specificity:
Do not react with mouse Fas/Fc Chimera、TNF-α、TRANCE,human APRIL、Fas Ligand、 TRAIL、VEGl etc.
تحویل:
یک ماه پس از سفارش
80400000
80400000 تومان