Solarbio

Mouse Dkk-3 ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative sandwich enzyme immunoassay technique. An antibody specific for mouse Dkk-3 has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and allow them to fully combine with the specific antibody. After washing away any unbound substances a horseradish peroxidase (HRP)-conjugated antibody specific for mouse Dkk-3 is added to detect the captured mouse Dkk-3 protein in the sample. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity proportional to the quantity of bound protein is then measurable at 450nm. You can calculate the concentration of mouse Dkk-3 in the samples by comparing the O.D. of the samples to the standard curve.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKM-0193-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

Reactivity:

Mouse

Detection Range:

7.81-500 pg/ml

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

3.9 pg/ml

Specificity:

In direct ELISA, cross-reactivity with recombinant Human (rh)DKK3 is about 20% and less than 1% with rhDKK1.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان