Mouse Cardiac Fibroblast Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal mice for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Throughout the entire tissue separation process, it is advisable to operate on ice. However, avoid freezing due to excessive cold; (3) The culture medium contains nutrients required for microbial growth. Please open it in a clean bench and aliquot, sealing the bottle opening with sealing film. Quick open and quick use, to avoid contamination; The kit is specifically designed for the isolation of primary mouse cardiac fibroblasts. It employs multi-step digestion and differential adhesion method to isolate and purify rat cardiac fibroblasts. Upon verification, following the standard operating procedures of this kit, one test can yield one bottle of cells (T25 culture flask), with a cell count exceeding 1×10? cells. Vimentin Immunofluorescence analysis confirms a cell purity exceeding 90%.