Mouse Brain Microvascular Endothelial Cell Isolation and Culture Kit

(1) Before the formal experiment, it is recommended to use 1-2 normal mice for anatomical simulation training to familiarize yourself with the operational procedures and improve tissue separation efficiency; (2) Throughout the entire tissue separation process, it is advisable to place the tissue-containing dishes on an ice tray/ice box (2-8°C) to maintain a low temperature. However, avoid freezing due to excessive cold, as this could cause tissue damage and impair extraction efficacy; (3) Reagent preparation or aliquoting must strictly follow aseptic technique. After aliquoting, immediately seal with a sealing film, use immediately, and avoid repeated freezing and thawing or contamination. The Mouse Brain Microvascular Endothelial Cell Isolation and Culture Kit is specifically designed for the isolation of primary mouse brain microvascular endothelial cells. Upon verification, following the standard operating procedures of this kit, one test can yield one bottle of cells (T25 culture flask), with a cell count exceeding 1×10? cells. When passaged at a 1:2 ratio, the cells can be passaged for 1–2 generations. Immunofluorescence analysis confirms a cell purity (CD31 positivity rate) exceeding 90%.