Monodehydroascorbate Reductase(MDHAR) Activity Assay Kit

Determination of Significance: MDHAR catalyzes MDHA to form AsA, which plays an important role in ascorbic acid redox metabolism. Measurement Principle: NADH reduces MDHA to generate AsA and NAD+ under the conditions of MDHAR catalysis. NADH has a characteristic absorption peak at 340 nm, but NAD+ is not. The activity of MDHAR can be calculated by measuring the decrease rate of absorption at 340 nm. Self Provided: Mortar/homogenizer, ice, desk centrifuge, spectrophotometer/microplate reader, micro quartz cuvette/96 well flat-bottom UV plate, adjustable pipette and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.