Mitochondrial complex III / CoQ-cytochrome C reductase Activity Assay Kit

Determination of Significance: Mitochondrial complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely found in mitochondria of animals, plants, microorganisms and cultured cells. It is a common component of the main pathway and branch of the mitochondrial respiratory electron transport chain. Mitochondrial complex III is responsible for transferring hydrogen from reduced CoQ to cytochrome C and then produce reduced cytochrome C. Measurement Principle: Unlike oxidized cytochrome C, reduced cytochrome C has a characteristic absorption at 550 nm, so the rate of increase in light absorption at 550 nm can reflect mitochondrial complex III enzyme activity. Self Provided: Spectrophotometer, water bath, desk centrifuge, transferpettor, mortar/homogenizer/cell ultrasonic crusher, 1ml glass cuvette, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.