Middlebrook 7H9 Broth Base

Preparation Method: Weigh 4. 7g of this product, add 0. 2ml glycerin every 90ml as appropriate in 900ml distilled water or deionized water, autoclaved at 121℃ for 15 minutes. Let cool at 45℃ and add 1 branch of ADC increasing bacteria solution（LA0270） to 180ml medium. Set aside. Composition: g/L Ammonium sulfate: 0. 5 Potassium dihydrogen phosphate: 1. 0 Disodium hydrogen phosphate: 2. 5 Sodium citrate: 0. 1 Magnesium sulfate: 0. 5 Ferric ammonium citrate: 0. 04 Zinc sulfate: 0. 001 Copper sulfate: 0. 001 L-Glutamate: 0. 5 Biotin: 0. 0005 Vitamin B6:0. 001 Calcium chloride: 0. 0005 pH: 6. 6±0. 2, 25℃ Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.