Micro Uric Acid (UA) Content Assay Kit

Determination of Significance: Uric acid is the end product of purine metabolism. Disorders of purine metabolism, energy metabolism and renal excretion of uric acid can cause the increase or decrease of plasma uric acid level. And then lead to a variety of diseases such as ventilation, kidney disease, cardiovascular disease. Therefore, the determination of uric acid content has an important guiding significance in clinical diagnosis. Measurement Principle: Uricase can catalyze the decomposition of uric acid into allantoin, CO2 and H 2 O 2 . Then Fe 2+ in potassium ferrocyanide is oxidized by H 2 O 2 to form Fe 3+ . Fe 3+ can further react with 4-aminoantipyrine and phenol to form red Quinones, which has a characteristic absorption peak at 505 nm. The content of uric acid can be calculated by measuring the absorbance value at 505 nm. Self Provided: Spectrophotometer/microplate reader, table centrifuge, water-bath, micro glass cuvette/96 well flat-bottom plate, adjustable pipette, mortar/ homogenizer, EP tube, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.