Mannitol Salt Agar

1.Due to varying microwave power, if the medium is not fully melted within 3 minutes, crush it into pieces before heating on high power, or extend the heating time by 0.5 to 1 minute.
2.Check for contamination. Do not use if microbial growth is observed.
3.Pour plates under aseptic conditions to prevent microbial contamination.
4.Do not overheat in the microwave, as excessive heating will cause severe water loss and reduce the nutritional performance of the product.
5.This product is for single use only. Pour out all the medium in the bottle and avoid repeated melting and solidification.
6.This product is not intended for clinical diagnosis. Test Principle: Peptone and beef extract provide carbon sources, nitrogen sources, vitamins and minerals. D-mannitol is a fermentable sugar. A high concentration of sodium chloride creates high osmotic pressure to inhibit most non-staphylococcal microorganisms. Phenol red acts as a pH indicator, and agar serves as the gelling agent for the medium. Typical pathogenic staphylococci (coagulase-positive) ferment D-mannitol to produce acid, forming yellow colonies with yellow halos. Typical non-pathogenic staphylococci cannot ferment D-mannitol and thus develop red colonies.