Malondialdehyde(MDA) Content Assay Kit

Determination of Significance: Lipid peroxide is produced by the action of oxygen free radicals on unsaturated fatty acid, then resolves to compounds, including malondialdehyde (MDA). The level of lipid peroxidation can be showed by detecting the level of MDA. Measurement Principle: Under acidic and high temperature conditions, the brown red 3,5,5- three methyl sulfamethoxazole -2,4-two ketone is synthesized with MDA and thiobarbituric acid (TBA) taking place condensation reaction, and the largest absorption wavelength is 532 nm. After colorimetry, the MDA content in the sample can be estimated. Self Provided: Spectrophotometer/microplate reader, water bath, centrifuge, transferpettor, micro glass cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.