Macrophages Depletion Reagent Set

Component: Clodronate liposomes 5ml, PBS liposomes 5ml Particle size: 200-400nm Product Introduction: Using the endocytosis mechanism of macrophages, clodronate, which is permeable to the membrane, is brought into the cell, and clodronate is released. When it reaches a certain concentration, it can induce apoptosis of macrophages, so as to achieve the purpose of removing macrophages. How it works: Amphiphilic phospholipids in water can form concentric circles of phospholipid bilayer spheres separated by water phase. The chlorophosphonate dissolved in aqueous solution is encapsulated in the water phase between the phospholipid bilayer during the process of liposome formation. Free clodronate cannot pass freely through the phospholipid bilayer of liposomes. As liposomes are engulfed by macrophages, clodronate dissolved in the water phase of liposomes can be gradually released and accumulated in cells under the action of macrophage lysosomal phosphonase. When it reaches a certain concentration, macrophages will be irreversibly damaged, thus entering the process of apoptosis. Clodronate released from dying macrophages has an extremely short half-life outside the cell (about 15 min) and can be eliminated outside the body via the urinary system.