Lipo9000 Plasmid Transfection Reagent (Universal)

Lipo9000 Plasmid Transfection Reagent (Universal) is suitable for transfecting plasmid DNA into various mammalian cell types. Lipo9000 Plasmid Transfection Reagent (Universal) exhibits exceptional transfection performance，efficiently transfecting multiple adherent and suspension cell types. In adherent cultured HeLa cells，Lipo9000 Plasmid Transfection Reagent (Universal) achieves an EGFP transfection positivity rate exceeding 90%. Notably，Lipo9000 Plasmid Transfection Reagent (Universal) also achieves high transfection efficiency in challenging cell types such as macrophages，monocytes，neural cells，and leukemia cells. In mouse RAW264.7 monocyte-macrophage cells，it achieves a transfection positive rate exceeding 50%. Formulated with milder，biodegradable biomaterials，Lipo9000 Plasmid Transfection Reagent (Universal) exhibits significantly lower cytotoxicity than Lipo Liposome 5000 Plasmid Transfection Reagent. Post-transfection cell death rates remain around 5%，enabling efficient transfection even in primary cells. Lipo9000 Plasmid Transfection Reagent (Universal) offers streamlined experimental procedures. No medium change is required 4-6 hours post-transfection，and researchers need not purchase Opti-MEM separately when preparing transfection complexes. The Lipo9000 Plasmid Transfection Reagent (Universal) kit includes Trans Buffer，which not only fully replaces Opti-MEM or serum-free media but also effectively reduces immune rejection reactions at the cell membrane. This significantly enhances plasmid DNA transfection efficiency and promotes protein expression，making it an ideal choice for DNA transfection experiments. Features: 1. Cell Transfection Performance: Equipped with Trans Buffer，it fully replaces Opti-MEM or serum-free media，effectively reduces immune rejection reactions of cell membranes，significantly enhances plasmid DNA transfection efficiency，and promotes protein expression. It efficiently transfects the vast majority of adherent cells，primary cells，and suspension cells. In adherent cultured Hela cells，the transfection positivity rate for EGFP can reach over 90%. 2. Exceptional plasmid DNA transfection efficiency: Demonstrates high transfection efficiency even in challenging cell types such as macrophages，monocytes，neural cells，hepatocellular carcinoma cells，gastric cancer cells，and leukemia cells. Achieves a transfection positivity rate exceeding 50% in mouse macrophage RAW264.7 cells. 3. Extremely low cytotoxicity: Utilizes an upgraded degradable biomaterial formulation with enhanced mildness，resulting in post-transfection cell mortality of only approximately 5%. This significantly reduces the impact of cytotoxicity on experimental outcomes; 4. Simple operation: Eliminates the need for medium change approximately 4-6 hours post-transfection. No additional purchase of Opti-MEM is required，and serum-containing medium can be used normally.