Lecithin Tween 80 Nutrient Agar

It is used for the determination of the total number of bacteria in cosmetics. Composition (g/L) Peptone 20. 0 Sodium chloride 5. 0 Beef soaked in flour 3. 0 Lecithin 1. 0 Twain 80 7. 0 Agar 15. 0 pH 7. 2±0. 1 25℃ Test Principle: Peptone and beef infusion powder provide carbon and nitrogen sources, vitamins and growth factors; Sodium chloride maintains a balanced osmotic pressure; Lecithin and Tween 80 can neutralize preservatives and play a role in dispersing substances in the emulsion, lecithin can neutralize quaternary ammonium salts, Tween 80 can neutralize phenol, hexachlorophenol, formalin, and the combination of the two can neutralize ethanol; Agar is a coagulant of the culture medium. Usage: weigh 48. 0g of this product, heat and boil to dissolve in 1000ml distilled water, divide and autoclave at 121 °C for 20 minutes, and set aside.