Hydroxymethylglutaryl Coenzyme A Synthase Activity Assay Kit

Determination of Significance: The mevalonate pathway is a very important metabolic pathway involved in the synthesis of precursors of many important terpenoids and is an important pathway in terpenoid biosynthesis. Hydroxymethylglutaryl coenzyme A synthase catalyzes the formation of acetyl coenzyme A and acetoacetyl coenzyme A to produce hydroxymethylglutaryl coenzyme A, a key step in the biosynthesis of cholesterol and isoprenoids. Measurement Principle: Hydroxymethylglutaryl coenzyme A synthase catalyzes the formation of hydroxymethylglutaryl coenzyme A from acetyl coenzyme A and acetoacetyl coenzyme A. It also produces CoASH, which converts DTNB to TNB (yellow), with characteristic absorbance values at 412 nm. Self Provided: Spectrophotometer/ microplate Reader, low temperature centrifuge, adjustable transferpettor, water bath/ constant temperature incubator, micro glass cuvettes/96 well plates, mortar/homogenizer/cell ultrasonicator , ice and distilled water. Note: Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.