Hydrogen Peroxide (H2O2) Content Fluorometric Assay Kit

H2O2 is the most common reactive oxygen molecules in organisms. It is mainly produced by the catalyzation of SOD and XOD and degraded by the catalyzation of CAT and POD. H2O2, which is not only one of the important reactive oxygen, but also the hub of mutual conversion of reactive oxygen. On the one hand, H2O2 can directly or indirectly oxidize intracellular nucleic acids, proteins and other biological macromolecules, and damage cell membranes, thus accelerating the aging and disintegration of cells. On the other hand, H2O2 is also a key regulatory factor in many oxidative emergency reactions. H2O2 reacts with the fluorescent probe under the action of enzyme to generate a strong fluorescent product, and its fluorescence intensity at the excitation wavelength of 535nm and emission wavelength of 587nm is proportional to the content of H2O2.