کیت
Solarbio

Human VE-Cadherin ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for VE-Cadherin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any VE-Cadherin present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for VE-Cadherin is added to detect the captured VE-Cadherin protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0619-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃,6 months
Assay Time:
3h
Reactivity:
Human
Detection Range:
468.75-30000 pg/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
234.38 pg/ml
Specificity:
Do not react with human β-Catenin Cadherin-6/Fc Chimera Cadherin-8/Fc Chimera Cadherin-11/Fc Chimera Cadherin-17/Fc Chimera CD31 E-Cadherin/FcChimera M-Cadherin/Fc Chimera N-Cadherin/Fc Chimera PARD-3 P-Cadherin/Fc Chimera PTPRB mouse E-Cadherin/Fc Chimera etc.
تحویل:
یک ماه پس از سفارش
100200000
100200000 تومان