کیت
Solarbio

Human Serpin A1 ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Serpin A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Serpin A1 present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Serpin A1 is added to detect the captured Serpin A1 protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0623-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃,6 months
Assay Time:
3h
Reactivity:
Human
Detection Range:
46.88-3000 pg/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
23.44 pg/ml
Specificity:
Do not react with human Serpin A3 Serpin A4 Serpin A5 Serpin A9 Serpin A11 Serpin C1 Serpin F2 mouse Serpin A1 Serpin D1 etc.
تحویل:
یک ماه پس از سفارش
80400000
80400000 تومان