کیت
Solarbio

Human IL-1F9 ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative sandwich enzyme immunoassay technique. An antibody specific for human IL-1F9/ IL-36 γ has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and allow them to fully combine with the specific antibody. After washing away any unbound substances a horseradish peroxidase (HRP)-conjugated antibody specific for human IL-1F9/ IL-36 γ is added to detect the captured human IL-1F9/ IL-36 γ protein in the sample. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity proportional to the quantity of bound protein is then measurable at 450nm. You can calculate the concentration of human IL-1F9/ IL-36 γ in the samples by comparing the O.D. of the samples to the standard curve.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0003-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
3h
Reactivity:
Human
Detection Range:
37.5-2400 pg/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
18.75 pg/ml
Specificity:
Do not react with Human IL-1RAcP/Fc Human Chimera Human IL-1Rrp2/FcChimera Human IL-36α(aa6-158) Human IL-36β(aa5-157) Human IL-36Ra Mouse IL-36γ etc.
تحویل:
یک ماه پس از سفارش
100200000
100200000 تومان