کیت
Solarbio

Human IL-17(High Sensitivity) ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-17/17A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any IL-17/17A present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for IL-17/17A is added to detect the captured IL-17/17A protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0026(HS)-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
3.5h
Reactivity:
Human
Detection Range:
0.625 pg/ml-40 pg/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
0.3 pg/ml
Specificity:
Do not react with Human IL-17B,IL-17B R,IL-17C,IL-17D ,IL-17E,Mouse IL-17,IL-17A ,IL-17B,IL-17BR,IL-17C ect.
تحویل:
یک ماه پس از سفارش
130200000
130200000 تومان