Solarbio

Human IL-12p70(One-Step）ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Human IL-12p70 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Human IL-12p70 present is captured by the coated antibody after incubation. A biotin-conjugate antibody specific for Human IL-12p70 is added to detect the captured Human IL-12p70 protein in the sample. Horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKH-0021(OS)-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

60min

Reactivity:

Human

Detection Range:

62.5-4000 pg/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

31.25 pg/ml

Specificity:

Do not react with Human G-CSF IL-6 IL-6Rα IL-12/IL-23 p40 IL-12 Rβ1 IL-12 Rβ2 IL-23 IL-27 ect.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان