Solarbio

Human IgG(Uncoated) ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IgG(Total) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any IgG(Total) present is captured by the coated antibody after incubation. After washing away any unbound substances, a HRP-conjugate antibody specific for IgG(Total) is added to detect the captured IgG(Total) protein in the sample. After extensive washing, a tetrameth_x0002_yl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKUH-0001-2*96T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Reactivity:

Human

Detection Range:

0.31-20 ng/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

150pg/ml

Specificity:

Do not react with rabbit IgG, mouse IgG, rat IgG, bovine IgG, human IgA or human IgM

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

2*96T

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