Solarbio

Human IgE ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative sandwich enzyme immunoassay technique. An antibody specific for human IgE has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and allow them to fully combine with the specific antibody. After washing away any unbound substances, a horseradish peroxidase (HRP)-conjugated antibody specific for human IgE is added to detect the captured human IgE protein in the sample. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm. You can calculate the concentration of human IgE in the samples by comparing the O.D. of the samples to the standard curve.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKH-0717-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

3.5h

Reactivity:

Human

Detection Range:

0.23-15 ng/ml

Recovery:

80%-120%

Sample Type:

Serum, plasma, Cell culture supernatant

Precision:

Coefficient of variation is < 10%

Sensitivity:

0.11 ng/ml

Specificity:

No cross-reactivity was observed with human IgA, human IgM, etc.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

80400000 تومان