کیت
Solarbio

Human Ferritin ELISA Kit

توضیحات و پروتکل‌های فنی

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Ferritin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells; any Ferritin present is captured by the coated antibody after incubation. After washing away any unbound substances, a biotin-conjugate antibody specific for Ferritin is added to detect the captured Ferritin protein in the sample. Following a wash to remove any unbound combination, horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing, a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. Solution containing sulfuric acid is used to stop color development. The color intensity, proportional to the quantity of bound protein, is then measurable at 450nm.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0620-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃,6 months
Assay Time:
3h
Reactivity:
Human
Detection Range:
93.75-6000 pg/ml
Recovery:
80%-120%
Sample Type:
Serum, plasma, Cell culture supernatant
Precision:
Coefficient of variation is < 10%
Sensitivity:
46.88 pg/ml
Specificity:
Do not react with human CD163 SCARA5 TFR-1 etc.
تحویل:
یک ماه پس از سفارش
100200000
100200000 تومان