کیت
Solarbio

Human E2 ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative competitive enzyme immunoassay (competitive ELISA) technique. An human E2 antigen has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately then add a HRP-conjugated antibody specific for human E2 to allow competitive binding between soluble human E2 in the samples/standards and the plate-coated human E2 for the limited antibody binding sites. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity inversely proportional to the concentration of human E2 is then measurable at 450nm. You can calculate the concentration of human E2 in the samples by comparing the O.D. of the samples to the standard curve.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0550-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
1h
Reactivity:
Human
Detection Range:
31.25-2000 pg/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
13.22 pg/ml
Specificity:
Can detect Human E2 in the sample, and there is no significant crossover with other related proteins.
تحویل:
یک ماه پس از سفارش
82800000
82800000 تومان