کیت
Solarbio

Human CA12 ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative sandwich enzyme immunoassay technique. An antibody specific for human CA12 has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately and allow them to fully combine with the specific antibody. After washing away any unbound substances a horseradish peroxidase (HRP)-conjugated antibody specific for human CA12 is added to detect the captured human CA12 protein in the sample. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity proportional to the quantity of bound protein is then measurable at 450nm. You can calculate the concentration of human CA12 in the samples by comparing the O.D. of the samples to the standard curve.
مشخصات و اطلاعات آزمایشگاهی
کد کاتالوگ:
C-SEKH-0116-48T
وضعیت تامین:
موجود در انبار سیترا بیوتک
Storage:
2-8℃ 6 months
Assay Time:
3h
Reactivity:
Human
Detection Range:
7.81-500 pg/ml
Recovery:
80%-120%
Sample Type:
Serum Plasma Cell Culture Supernatant
Precision:
Both intra-plate and inter-plate variation coefficients were less than 10%
Sensitivity:
4 pg/ml
Specificity:
Do not react with Human CA2 Human CA3 Human CA4 Human CA5A Human CA5B Human CA8 Human CA10 Human CA13 Human CA14 Human CA9 Mouse CA12 etc.
تحویل:
یک ماه پس از سفارش
80400000
80400000 تومان