Human Airway Organoid Medium

Aliquoted organoid media should be stored at -20℃ for two years to avoid repeated freeze-thaw cycles. The thawed medium can be stored at 2-8℃ and is recommended to be used within two weeks. Organoid media contain bacterial and fungal antibiotics. The organoid medium should be thawed in a four-degree freezer, the thawed medium should be mixed upside down and mixed thoroughly, and the medium should be aliquoted in a sterile biosafety cabinet or clean bench, 10ml aliquots are recommended. Store the aliquot medium at -20 ℃, take out the aliquot medium and thaw it before use. The Human-Tube-Centric Organoid Medium Set is a chemically defined cell culture medium for the establishment and maintenance of human-tube-based organoids derived from human tracheal stem cells. The self-renewing epithelial cells of the trachea are driven by the proliferation of basal cells. Humanescent organoids contain basal cells, ciliated cells, secretory cells, and a small number of neuroendocrine cells, so organoids show all the characteristics of the trachea. Epithelial cells have great applications in terms of structure, cell type composition, and self-renewal dynamics, and can be used for the study of tracheal homeostasis and diseases.