Solarbio

HIS ELISA Kit

توضیحات و پروتکل‌های فنی

Solarbio ELISA kit uses the quantitative competitive enzyme immunoassay (competitive ELISA) technique. An HIS antigen has been pre-coated onto a microplate; Add gradient-diluted standards and pre-diluted samples separately then add a biotin-conjugated antibody specific for HIS to allow competitive binding between soluble HIS in the samples/standards and the plate-coated HIS for the limited antibody binding sites. After washing away any unbound substances horseradish peroxidase (HRP)-conjugated Streptavidin is added to the wells. After extensive washing a tetramethyl-benzidine (TMB) reagent is added to the wells for signal development. A Solution containing sulfuric acid is used to stop color development. The color intensity inversely proportional to the concentration of HIS is then measurable at 450nm. You can calculate the concentration of HIS in the samples by comparing the O.D. of the samples to the standard curve.

مشخصات و اطلاعات آزمایشگاهی

کد کاتالوگ:

C-SEKSM-0029-48T

وضعیت تامین:

موجود در انبار سیترا بیوتک

Storage:

2-8℃ 6 months

Assay Time:

1.25h

Reactivity:

Common Species

Detection Range:

1.563-100 ng/ml

Recovery:

80%-120%

Sample Type:

Serum Plasma Cell Culture Supernatant

Precision:

Both intra-plate and inter-plate variation coefficients were less than 10%

Sensitivity:

0.94 ng/ml

Specificity:

Can detect HIS in the sample, and there is no significant crossover with other related proteins.

تحویل:

یک ماه پس از سفارش

انتخاب مقیاس / حجم بسته‌بندی:

48T 96T

95400000 تومان